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新聞資訊 / news 您的位置:網站首頁 > 新聞資訊 > NanoView外泌體檢測系統榮獲“2021生命科學十·大創新產品”

NanoView外泌體檢測系統榮獲“2021生命科學十·大創新產品”

發布時間: 2022-02-28  點擊次數: 718次

生物通主辦的“2021生命科學十·大創新產品”于2022年1月正式揭曉,Quantum Design中國·新引進的NanoView R200全自動外泌體熒光檢測分析系統,憑借其獨特的設計理念及卓·越的性能,榮獲“2021生命科學十·大創新產品”!

 

 

 

 

“生命科學十·大創新產品”是生物通歷經10余年打造的生命科學科學儀器行業最·先進、創新的獎項之一。至今已舉辦十四屆。本次評選共有28臺儀器參選,經過評審專家嚴格篩選以及廣大科研用戶的投票評比,最終評選出10·大創新產品。該獎項致力于讓大家了解生命科學發展的新方向,也希望新穎的產品能激發研究者的靈感,推動科研進程,助力科研學者早日攀上科學研究的高峰!

 


 

全自動外泌體熒光檢測分析系統ExoView是美國NanoView Biosciences于2018年研究推出的,該系統一經推出,便引起了外泌體領域科研工作者的廣泛關注。僅兩年已有100多個實驗室采用該技術,發表文獻近篇,刊載在Cell, Cell Biology, Journal of Extracellular Vesicles等期刊。ExoView的精準表征,為科學家提供了一種無須純化即可直接對血漿、血清、尿液、培養基中的外泌體進行表征分析的新方法。能夠提供單個外泌體水平下的全·方位外泌體表征信息(包括粒徑大小、分布、計數、攜帶蛋白表達與生物標志物(CD9,CD81,CD63等)共定位等),以及多層次和全方面的外泌體測量解決方案。能夠幫助科學家更深入地了解外泌體與疾病之間的關系,助力疾病診斷和新藥開發。

 

 

 

ExoView® R200全自動外泌體熒光檢測分析系統優勢包括:


• 特異性捕獲:芯片上可包被多達6 種捕獲抗體,特異性捕獲含特定蛋白標記物的外泌體。

 

 

• 絕對陽性外泌體計數:芯片捕獲外泌體后,可通過單粒子干涉反射成像傳感器(SP-IRIS)技術直接檢測樣品中外泌體的數量。

 

 

 

• 單個外泌體蛋白共定位分析:檢測每個外泌體的熒光信號并進行統計,可獲得熒光共定位信息,用于分析樣品中不同表型外泌體的比例。

 

 

• 無需純化:使用抗體捕獲模式,防止樣品中雜質影響結果,可直接檢測血液、尿液和細胞培養液中的外泌體,未純化樣品的測量結果與純化后基本一致。

 

 

• 粒徑分辨率高:高精度SP-IRIS技術,可檢測≥50 nm的外泌體,測量結果與電子顯微鏡檢測結果基本一致,并統計生成外泌體的粒徑分布結果。

 

 

• 可檢測外泌體內容物:試劑盒配套相應的穿膜劑,可穿透外泌體并對外泌體內容物進行染色并檢測,如下圖所示,Non-permeabilized時只能檢測到跨膜蛋白CD9的熒光信號, Permeabilized后即可檢測到外泌體內容物(如PD-L1、P-tau等)的表達。

 

 

 

部分應用單位:

 

 

部分發表文獻:

• Andras Saftics.(2021) Data evaluation for surface-sensitive label-free methods to obtain real-time kinetic and structural information of thin films: A practical review with related software packages. Advances in Colloid and Interface Science.

• Kyoung-Won Ko.(2021) Integrated Bioactive Scaffold with Polydeoxyribonucleotide and Stem-Cell-Derived Extracellular Vesicles for Kidney Regeneration. ACS Nano.

• Tanina Arab. (2021) Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms. Journal of Extracellular Vesicles.

• Niaz Z.Khan.(2021) Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential. Brain, Behavior, and Immunity.

• Dario Brambilla. (2021) EV Separation: Release of Intact Extracellular Vesicles Immunocaptured on Magnetic Particles. Analytical Chemistry.

• Enkhtuya Radna. (2021) Extracellular vesicle mediated feto-maternal HMGB1 signaling induces preterm birth. Lab on a Chip.

• Li, M., Soder. (2021) WJMSC‐derived small extracellular vesicle enhance T cell suppression through PD‐L1. Journal of Extracellular Vesicles.

• Crescitelli, R. (2021) Isolation and characterization of extracellular vesicle subpopulations from tissues. Nature protocols.

• Berger, A. (2021). Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula. Nanoscale.

• Vidal, M. (2020) Exosomes and GPI-anchored proteins: Judicious pairs for investigating biomarkers from body fluids. Advanced drug delivery reviews.

• K Cho, H Kook.(2020)Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy. Journal of Extracellular Vesicles.

• Maximillian A. Rogers.(2020)Annexin A1–dependent tethering promotes extracellular vesicle aggregation revealed with single–extracellular vesicle analysis. Cell Biology.

• Annette M. Marleau.(2020)Targeting tumor-derived exosomes using a lectin affinity hemofiltration device. Cancer Research.

• Alessandro Gori.(2020)Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis. Journal of Extracellular Vesicles.

• Rossella Crescitelli.(2020)Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation. Journal of Extracellular Vesicles.

• Maria S. Panagopoulou.(2020) Phenotypic analysis of extracellular vesicles:  a review on the applications of fluorescence. Journal of Extracellular Vesicles.

• WeiYan.(2020) Immune Cell-Derived Exosomes in the Cancer-Immunity Cycle. Trends in Cancer.

• Daniel Bachurski. (2019) Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation. Cell.

 

為了更好的服務中國客戶,Quantum Design中國子公司在北京建立了專業的客戶服務中心,正式推出專業的全·方位外泌體表征測試服務,您只需要少量樣品即可獲得全·方位的外泌體表征數據。

 

 

歡迎各位老師垂詢:010-85120280。前·10名訂購服務的老師,可享受8折優惠!

 

掃描上方二維碼,即刻訂購吧!

 

相關產品:

1、全自動外泌體熒光檢測分析系統

https://www.chem17.com/product/detail/36729984.html

 

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